Overview
Using the Nanotrap® Microbiome B Particles to concentrate bacteria from wastewater samples from two rural wastewater treatment plants (WWTP), the Hawaii State Department of Health (HDOH) State Laboratories Division (SLD) performed studies of both temporal distribution and genetic diversity of AMR genes found within two Hawaiian communities.
Results
Nanotrap Microbiome B Particles were capable of concentrating bacteria containing each of the AMR targets at different points throughout the 13-week period (Fig 1). Within this period, KPC-1 and OXA-48 genes were prevalent within wastewater samples from both WWTPs, with the highest genomic presence of KPC-1. The NDM and IMP-1 genes were consistently detected from WWTP 1, while they were less detectable from WWTP 2 over the period. VIM-1 was detected from both WWTPs at low concentrations, and different points throughout the period.
When the same samples were sequenced, the data showed large abundance values for both the KPC and OXA beta-lactamase genes, correlating with the dPCR data. NDM, IMP and VIM beta-lactamase genes were observed across the two WWTPs, but at much lower abundance. Wastewatern two separate Hawaiian islands. from the two WWTPs was found to show a similar AMR genetic diversity while being located on two separate Hawaiian islands.
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