Nanotrap Protein Enrichment Affinity Kits for Protein Enrichment and Biomarker Discovery from Cerebrospinal Fluid Samples

App Note -- Nanotrap PEAK for Protein Enrichment and Biomarker Discovery from Cerebrospina

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Mass-spectrometry-based proteomic analysis of biological samples is a powerful tool for the identification of potential biomarkers. Cerebrospinal fluid (CSF) is a common sample type used in proteomic analyses because of its clinical relevance and low protein complexity. However, high abundances of albumin and immunoglobin proteins can mask the detection of lower abundance proteins. (1)

In this application note, we demonstrate how to use the Nanotrap® Protein Enrichment Affinity Kits (PEAK) to manually process CSF samples. We compare the number of unique protein identifications obtained from a CSF sample using different Nanotrap Protein Particle workflows to the same CSF sample processed without using the Nanotrap PEAK. We also compare the overlap of unique protein identifications for these different workflows.

The Venn diagram illustrates the overlap of unique protein identifications (protein IDs) for the 1-particle Method using each of the three different Nanotrap Protein Particle types.

APPLICATION NOTE SKU # 34XXX

Lit. # CSF-AN31394