The study objective is to detect and quantify the proportion of SARS-CoV-2 variants in wastewater utilizing a genotyping assay designed by Applied Biosystems™ TaqMan™ SARS-CoV-2 Mutation Research Panel on either Digital PCR or Real-time qPCR platforms.
Over eight assays and more than two hundred wastewater samples were evaluated using both Real-Time qPCR and Digital PCR as part of validation process. Genotyping assays were able to identify variant transition phases in wastewater.
The study is funded by the National Institute of Health with collaboration between ROSALIND, Inc., Ceres Nanosciences, Inc., and Emory University. Emory University initiated the application of the Thermo Fisher SARS-CoV-2 variant detection assays to wastewater samples and developed this SOP. Ceres reviewed and revised the SOP.
Study Protocol SKU 44XXX SKU 10XXX
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