ABSTRACT: Methods of wastewater concentration (electronegative filtration (ENF) versus magnetic bead-based Magnetic Bead-Based Concentration and V2G-qPCR to RT-qPCR for Quantifying Viral SARS-CoV-2 RNA from Wastewater

medRxiv May 2023 Background The origin of divergent SARS-CoV-2 spike sequences found in wastewater, but These “cryptic” wastewater sequences have harbored many of the same mutations that later emerged in Omicron We first detected a cryptic lineage in municipal wastewater in Wisconsin in January 2022. Findings The persistence of the Wisconsin Lineage signal allowed us to trace it from a central wastewater Interpretation We propose that prolonged detection of the Wisconsin Lineage in wastewater represents

APPLICATION NOTE SKU 10XXX SKU 44XXX Download full application note here Key Advantages > Enables detection Omicron, Delta) in wastewater samples.

"Intensity of sample processing methods impacts wastewater SARS-CoV-2 whole genome amplicon sequencing outcomes" Science of The Total Environment, June 2023 ABSTRACT: Wastewater SARS-CoV-2 surveillance has Genomic surveillance of SARS-CoV-2 in wastewater, particularly the efforts for whole genome sequencing The intrinsic sample limitations are inherent to wastewater. samples, played important roles in wastewater SARS-CoV-2 amplicon sequencing.

bioRxiv, February 2022 ABSTRACT: Monitoring wastewater samples at building-level resolution screens large Here we perform untargeted metatranscriptomics on virally-enriched wastewater samples from 10 locations emergent threats through changes in the human microbiome, suggesting new approaches for untargeted wastewater-based

APPLICATION NOTE SKU 44XXX Download full application note here MACHERY NAGEL: SARS-CoV-2 extraction from wastewater concentrated with Nanotrap® Magnetic Virus Particles Automatable concentration and purification of viral RNA from wastewater Introduction Many researchers, private companies, and public health agencies Viral RNA extraction from wastewater is a major challenge for laboratories due to the presence of so many substances that cause inhibition of downstream analysis, and because large volumes of wastewater

SARS-CoV-2 RNA concentration in wastewater successfully tracks regional infection dynamics and provides Tracking virus genomic sequences in wastewater would improve community prevalence estimates and detect However, two factors limit wastewater-based genomic surveillance: low-quality sequence data and inability Here, we resolve these critical issues to perform a high-resolution, 295-day wastewater and clinical Our study provides a scalable solution for wastewater genomic surveillance that allows early detection

Morbidity and Mortality Weekly Report, February 2023 Notes from the Field: Aircraft Wastewater Surveillance Because SARS-CoV-2 can be detected in feces and urine of some infected persons (2), wastewater surveillance Sampling wastewater directly from aircraft can be used to link SARS-CoV-2 lineage data with flight origin

"Wastewater Surveillance of U.S. A pilot study of wastewater testing was initiated in February, 2021 to determine its feasibility as a Wastewater was analyzed for the presence of two viral genes, N and E, and quantified relative to levels Wastewater data was validated by comparison with concomitant screening and surveillance programs that strategic locations and is actively continuing its wastewater testing program.

Enterica, and crAssphage from wastewater without the need of filtration, bead-beating, or centrifugation Introduction Recently, wastewater surveillance has become widely recognized as a powerful tool to identify Many of the investments made to date have been focused on developing wastewater surveillance methods Moreover, it was recently demonstrated that Nanotrap particle processing of wastewater testing can enable Nanotrap particle methods have also enabled detection of monkeypox and hepatitis A virus in wastewater

APPLICATION NOTE SKU 10XXX SKU 44XXX Download full application note here Key Advantages >> Can be used for wastewater surveillance of multiple viruses, including SARS-CoV-2, Influenza A and B. > Detection Municipal wastewater harbors a great variety of pathogenic viruses. been conducted on the persistence of human enteric viruses, transmitted via the fecal-oral route, in wastewater Wastewater surveillance is appealing because a single sample can ‘test’ a large population, it can enable

In this application note, we show that Nanotrap® Microbiome A Particles with the MagMAX™ Wastewater Ultra Nucleic Acid Isolation Kit is compatible with the Clear Dx™ FlexPro: Wastewater (SARS-CoV-2) and leads Wastewater-based epidemiology (WBE) increased in prevalence during the pandemic as a way to measure disease Accurate and complete sequencing from wastewater requires clean and concentrated nucleic acid samples The Clear Dx FlexPro: Wastewater (SARS-CoV-2) WGS Reagent and Automation Bundle uses targeted sequencing