Wastewater-based Epidemiology
Nanotrap® Microbiome Particles rapidly concentrate microbes from raw sewage, requiring no filtration, centrifugation, or bead-beating.
Large-scale wastewater surveillance can be used to help communities monitor infection dynamics for SARS-CoV-2 and other microbes of concern. Robust, high-throughput methods based on Nanotrap Microbiome Particles are enabling wide-spread implementation.
A method using Nanotrap Microbiome A Particles was shown to detect 1 asymptomatic individual in a building of 415 residents and enabled early detection of nearly 85% of individual COVID-19 cases on a university campus.
Wastewater-based epidemiology workflow
Simplified workflow.
-
Eliminate filtration, centrifugation and bead beating methods, without sacrificing sensitivity.
-
Spend less time in the fume hood.
-
Shelf stable product for up to 6 years at room temperature — no need for cold storage.
Highly compatible.
-
Compatible with RT-qPCR, RT-ddPCR, RT-dPCR, and sequencing-based analysis methods.
-
Manual and automated protocols available for use with a wide range of RNA extraction kits.
Capture more. Get better data.
-
Improved capture efficiency with 2-10x higher recovery.
-
Need greater performance? Add a Nanotrap Enhancement Reagent to improve results by another 1-2 Ct values.
Environmentally friendly.
Less plastics = Less waste.
-
Reduce biohazard waste
by using less plastic consumables compared
to other methods. -
Free up valuable lab storage space.
Supported by the U.S. National Institutes of Health.
Poster: Surveillance of SARS-CoV-2 in wastewater of Ohio: Consolidation of SARS-CoV-2 testing from academic, commercial and government labs to Ohio Department of Health Laboratory
Application Notes
-
Capture and Concentrate Microbes that Cause Sexually Transmitted Infections from Wastewater
-
Capture and Concentrate Pathogenic Legionella Species from Environmental Water Samples
-
Nanotrap Microbiome A and B Particles can be combined to improve microbe capture from wastewater
-
Wastewater sequencing uncovers early, cryptic SARS-CoV-2 variant transmission
-
Comparison of Nanotrap Microbiome A Particles, membrane filtration, and skim milk workflows for SARS-CoV-2 concentration in wastewater
